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Serum was then seeded (5 L for IgM, 1 L for IgG or IgA measurements, respectively) into the well of a 96-deep-well plate (Beckman Coulter Inc.) and 25 L of the diluted antigen preparation was added, followed by a 2 h incubation at room temperature

15 For example, the supernatant extracted from a Lactobacillus rhamnosus GG culture reverses alcohol-induced intestinal permeability by restoring optimal levels of tight junction proteins and the factors involved in mucin production

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Neural activity results in increased production of ROS which have been linked to neurological disorders 20,21,22